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vip sirna  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology vip sirna
    Vip Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vip+sirna/VIP+siRNA/pmc06868285-183-9-11
    Average 90 stars, based on 4 article reviews
    vip sirna - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Trophoblast cells inhibit neutrophil extracellular trap formation and enhance apoptosis through vasoactive intestinal peptide-mediated pathways.
    Article Snippet: To obtain trophoblast CM, cells were cultured for 20 h in RPMI 2% FBS with or without 50 nM VIP antagonist (Bachem, CA, USA). .. Swan-71 cells were transfected with 50–100 nM VIP siRNA (Santa Cruz Biotechnology, Dallas, TX, USA): Lipofectamine RNAimax (Life Technologies, Grand Island) complex as previously described (Vota et al., 2016). ..

    Article Title: Vasoactive Intestinal Peptide induces glucose and neutral amino acid uptake through mTOR signalling in human cytotrophoblast cells
    Article Snippet: Cells were maintained in culture flasks at 37 °C, 5% CO 2 in Dulbeco’s modified Eagle’s medium and Nutrient Mixture F12 (DMEM-F12) (Life Technologies, Grand Islands, NY, US) supplemented with 10% heat-inactivated foetal bovine serum (FBS), 2 mM Glutamine (Sigma-Aldrich, Missouri, US) and 100 U.ml −1 streptomycin-100 μg.ml −1 penicillin solution (Life Technologies, Grand Islands, NY, US) , . .. Swan 71 and BeWo cells were transfected with a VIP siRNA (Santa Cruz Biotechnology, Dallas, TX, US) as previously described . ..

    Incubation:

    Article Title: Vasoactive intestinal peptide shapes first‐trimester placenta trophoblast, vascular, and immune cell cooperation
    Article Snippet: .. To transfect BeWo cells with a VIP siRNA (Santa Cruz Biotechnology, Dallas, TX, USA), cells were grown at 60% of confluence and arrested for 3 hr in Optimem®; 100 nM VIP siRNA: Lipofectamine RNAimax (Life Technologies, Grand Island, NY, USA) complex were made in Optimem and incubated for 15 min before being added to the cells in a drop wise manner; 24‐hr post‐transfection, the media were changed for DMEM:F12 supplemented with 10% FCS for another 48 hr. siRNA with a scramble (Scrl) sequence was used as a negative control. ..

    Article Title: Vasoactive intestinal peptide shapes first‐trimester placenta trophoblast, vascular, and immune cell cooperation
    Article Snippet: .. VIP silencing in trophoblast cell lines To transfect BeWo cells with a VIP siRNA (Santa Cruz Biotechnology, Dallas, TX, USA), cells were grown at 60% of confluence and arrested for 3 hr in Optimem®; 100 nM VIP siRNA: Lipofectamine RNAimax (Life Technologies, Grand Island, NY, USA) complex were made in Optimem and incubated for 15 min before being added to the cells in a drop wise manner; 24‐hr post‐transfection, the media were changed for DMEM:F12 supplemented with 10% FCS for another 48 hr. siRNA with a scramble (Scrl) sequence was used as a negative control. ..

    Sequencing:

    Article Title: Vasoactive intestinal peptide shapes first‐trimester placenta trophoblast, vascular, and immune cell cooperation
    Article Snippet: .. To transfect BeWo cells with a VIP siRNA (Santa Cruz Biotechnology, Dallas, TX, USA), cells were grown at 60% of confluence and arrested for 3 hr in Optimem®; 100 nM VIP siRNA: Lipofectamine RNAimax (Life Technologies, Grand Island, NY, USA) complex were made in Optimem and incubated for 15 min before being added to the cells in a drop wise manner; 24‐hr post‐transfection, the media were changed for DMEM:F12 supplemented with 10% FCS for another 48 hr. siRNA with a scramble (Scrl) sequence was used as a negative control. ..

    Article Title: Vasoactive intestinal peptide shapes first‐trimester placenta trophoblast, vascular, and immune cell cooperation
    Article Snippet: .. VIP silencing in trophoblast cell lines To transfect BeWo cells with a VIP siRNA (Santa Cruz Biotechnology, Dallas, TX, USA), cells were grown at 60% of confluence and arrested for 3 hr in Optimem®; 100 nM VIP siRNA: Lipofectamine RNAimax (Life Technologies, Grand Island, NY, USA) complex were made in Optimem and incubated for 15 min before being added to the cells in a drop wise manner; 24‐hr post‐transfection, the media were changed for DMEM:F12 supplemented with 10% FCS for another 48 hr. siRNA with a scramble (Scrl) sequence was used as a negative control. ..

    Negative Control:

    Article Title: Vasoactive intestinal peptide shapes first‐trimester placenta trophoblast, vascular, and immune cell cooperation
    Article Snippet: .. To transfect BeWo cells with a VIP siRNA (Santa Cruz Biotechnology, Dallas, TX, USA), cells were grown at 60% of confluence and arrested for 3 hr in Optimem®; 100 nM VIP siRNA: Lipofectamine RNAimax (Life Technologies, Grand Island, NY, USA) complex were made in Optimem and incubated for 15 min before being added to the cells in a drop wise manner; 24‐hr post‐transfection, the media were changed for DMEM:F12 supplemented with 10% FCS for another 48 hr. siRNA with a scramble (Scrl) sequence was used as a negative control. ..

    Article Title: Vasoactive intestinal peptide shapes first‐trimester placenta trophoblast, vascular, and immune cell cooperation
    Article Snippet: .. VIP silencing in trophoblast cell lines To transfect BeWo cells with a VIP siRNA (Santa Cruz Biotechnology, Dallas, TX, USA), cells were grown at 60% of confluence and arrested for 3 hr in Optimem®; 100 nM VIP siRNA: Lipofectamine RNAimax (Life Technologies, Grand Island, NY, USA) complex were made in Optimem and incubated for 15 min before being added to the cells in a drop wise manner; 24‐hr post‐transfection, the media were changed for DMEM:F12 supplemented with 10% FCS for another 48 hr. siRNA with a scramble (Scrl) sequence was used as a negative control. ..



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